Journal
/
Inmunología y contagio
/
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

DOI:
10.3791/56325-v

•

07:29 min

•

October 12, 2017

•

, , , , , ,
1University of California, Los Angeles, 2Centre for Biomedical Research,Burnet Institute, 3School of Health and Biomedical Sciences,RMIT University, 4Department of Infectious Diseases,Monash University

Capítulos

  • 00:05Título
  • 00:58Day 1: Preparation of Controls and Laboratory-specific Quality Controls
  • 02:10Day 1: Separation of HDL Cholesterol using HDL Precipitation
  • 03:07Day 2: Fluorochrome Assay
  • 05:17Results: Cell-free Biochemical Fluorometric Assay Results
  • 06:32Conclusion

Summary

Traducción Automática

Traducción Automática

We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

Tags

Cell-freeBiochemicalFluorometricEnzymatic AssayHigh-throughputLipid PeroxidationHigh Density LipoproteinHDL FunctionCardiovascular DiseaseMetabolic AbnormalitiesInflammatory DiseasesHDL-CHDLoxPooled Quality ControlHDL Cholesterol Precipitating ReagentHRPFluorochrome ReagentDMSO

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

High-throughput Detection Method for Influenza Virus

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

High-throughput Gene Tagging in Trypanosoma brucei

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Read Article