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Live Imaging of Mouse Secondary Palate Fusion
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Live Imaging of Mouse Secondary Palate Fusion
DOI:

06:10 min

July 27, 2017

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Capítulos

  • 00:05Título
  • 00:42Palate Explant Culture and Confocal Time-lapse Imaging
  • 03:28Results: Representative Live Palate Explant Culture Imaging
  • 05:16Conclusion

Summary

Traducción Automática

Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

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