Journal
/
Entwicklungsbiologie
/
Live Imaging of Mouse Secondary Palate Fusion
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Live Imaging of Mouse Secondary Palate Fusion

Live Imaging of Mouse Secondary Palate Fusion

DOI:
10.3791/56041-v

•

06:10 min

•

July 27, 2017

•

, ,
1Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics,University of California San Francisco, 2Laboratory of Transgenic Models Diseases,Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

Kapitel

  • 00:05Titel
  • 00:42Palate Explant Culture and Confocal Time-lapse Imaging
  • 03:28Results: Representative Live Palate Explant Culture Imaging
  • 05:16Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

Tags

Live ImagingMouseSecondary Palate FusionConfocal MicroscopyCranial Facial DevelopmentPalatal ShelvesMidline Epithelia SeamEpithelial ConvergenceExplant Culture

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Live-imaging of the Drosophila Pupal Eye

In Vitro Differentiation of Mature Myofibers for Live Imaging

Live Confocal Imaging of Developing Arabidopsis Flowers

Long-term Live Imaging of Drosophila Eye Disc

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Live Cell Imaging of Chromosome Segregation During Mitosis

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Read Article