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Biologia do Desenvolvimento
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Live Imaging of Mouse Secondary Palate Fusion
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Live Imaging of Mouse Secondary Palate Fusion

Live Imaging of Mouse Secondary Palate Fusion

7,394 Views

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06:10 min

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July 27, 2017

DOI:

10.3791/56041-v

DOI:
10.3791/56041-v

•

06:10 min
July 27, 2017

•

6 Views
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1Department of Cell and Tissue Biology, Program in Craniofacial Biology and Institute for Human Genetics,University of California San Francisco, 2Laboratory of Transgenic Models Diseases,Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences

Summary

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Here, we present a protocol for live imaging of mouse secondary palate fusion using confocal microscopy. This protocol can be used in combination with a variety of fluorescent reporter mouse lines, and with pathway inhibitors for mechanistic insight. This protocol can be adapted for live imaging in other developmental systems.

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Cite this Article

Kim, S., Prochazka, J., Bush, J. O. Live Imaging of Mouse Secondary Palate Fusion. J. Vis. Exp. (125), e56041, doi:10.3791/56041 (2017).

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