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Mäta Biomolekylär DSC profiler med termolabila ligander till snabbt karakterisera vikning och bindande interaktioner
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Bioquímica
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JoVE Revista Bioquímica
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
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Mäta Biomolekylär DSC profiler med termolabila ligander till snabbt karakterisera vikning och bindande interaktioner

8,085 Views

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09:15 min

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November 21, 2017

DOI:

10.3791/55959-v

DOI:
10.3791/55959-v

•

09:15 min
November 21, 2017

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2 Views
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1Department of Chemistry,McGill University, 2Department of Chemistry,York University

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Biomolecular FoldingBinding InteractionsDifferential Scanning Calorimetry (DSC)Thermolabile LigandThermodynamic ParametersBiocalorimetryDrug DesignLigand TitrationDialysisBuffer MatchingBiomolecule ConcentrationDegassingDSC Setup

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Harkness V, R. W., Johnson, P. E., Mittermaier, A. K. Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions. J. Vis. Exp. (129), e55959, doi:10.3791/55959 (2017).

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