Journal
/
Genética
/
逆转录病毒扫描：映射MLV集成站点以定义细胞特异性监管区域
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Please note that all translations are automatically generated. Click here for the English version.

逆转录病毒扫描：映射MLV集成站点以定义细胞特异性监管区域

DOI:
10.3791/55919-v

•

10:10 min

•

May 28, 2017

•

, , , , , , ,
1Center for Genome Research, Department of Life Sciences,University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development,Imagine Institute, 3Institute for Biomedical Technologies,CNR, 4Généthon, 5Sorbonne Paris Cité – Université Paris Descartes

Capítulos

  • 00:05Título
  • 00:45MLV Transduction of Human Cells
  • 01:41Restriction Enzyme Digestion and Linker Ligation
  • 03:05First-round Linker-mediated PCR
  • 03:43Second Round Linker-mediated PCR
  • 05:17Library Preparation and Dilution for Massive Sequencing of MLV Integration Sites
  • 07:53Results: Mapping MLV Integration into Epigenetically-defined Regulatory Regions
  • 09:23Conclusion

Summary

Traducción Automática

Traducción Automática

在这里，我们描述了人类细胞中基于Moloney小鼠白血病病毒的逆转录病毒载体的整合位点全基因组图谱的方案。

Tags

Retroviral ScanningMLV Integration SitesCell-specific Regulatory RegionsGene TherapyGene RegulationMLV-derived Retroviral VectorEGFP Reporter GeneFlow Cytometric AnalysisGenomic DNA PreparationRestriction Enzyme DigestionLinker LigationFirst-round PCRSecond-round PCR

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

QTL定位和CRISPR / Cas9编辑识别抗药性基因<em>弓形虫</em

使用生物素化补骨脂素全球定位RNA-RNA相互作用

双向逆转录病毒整合位点PCR方法和定量数据分析工作流程

G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法

甲醛辅助分离测定哺乳动物细胞染色质可及性的调控元素

一种快速、定量的后向基因组转化和变异的多肽映射方法

一种新的饱和诱变方法: 利用 Phosphorothioates 对 RNA 调控蛋白结合位点的单步表征

利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列

单液滴数字聚合酶链反应对热点地区突变的综合和同步检测

探索序列空间,识别调节RNA结合蛋白的结合位点

Read Article