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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
 

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Article DOI: 10.3791/55893-v • 06:32 min • June 5th, 2017
June 5th, 2017 •
1, 1,2, 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

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Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

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Immunostaining Olfactory Bulb Olfactory Sensory Axon Molecular Identity Codes Axon Sorting Molecules Immunohistochemical Staining Method Olfactory Development Axon Guidance Synapse Formations Regeneration Of Olfactory Sensory Neurons Expression Patterns Embedding Step Visual Demonstration Mice Paraformaldehyde Lower Jawbone Olfactory Epithelium Nasal Cavity Brain Embedding Mold
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