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Analisi della concorrenza oligopeptide per la determinazione del sito di fosforilazione

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Oligopeptide Competition Assay for Phosphorylation Site Determination

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Analisi della concorrenza oligopeptide per la determinazione del sito di fosforilazione

8,275 Views

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09:16 min

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May 18, 2017

DOI:

10.3791/55708-v

DOI:

10.3791/55708-v

•

09:16 min

May 18, 2017

•

1 Views

Min Sung Joo, Ja Hyun Koo, Sol-Bi Shin, Hyungshin Yim, Sang Geon Kim

¹College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, ²College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Tags

Oligopeptide Competition Assay Phosphorylation Site Determination Cell Signaling Post-translational Modifications Site-directed Mutagenesis PCR DpnI Digestion Bacterial Transformation DNA Sequencing Protein Expression And Purification

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