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Biochemistry

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JoVE Journal Biochemistry
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
 

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Article DOI: 10.3791/55011-v • 07:41 min • February 4th, 2017
February 4th, 2017 •
1,2, 3, 4, 4, 3, 1,2
1Department of Medicine, Unit of Computational Medicine, Karolinska Institutet, 2Center for Molecular Medicine, Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2, Karolinska Institutet, 4Department of Medicine, University of California San Diego

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This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

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Metabolite Extraction Cell Sorting Stable Isotope Tracing Metabolism Cell Subpopulations Cell Culture Mass Spectrometry
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