Journal
/
Bioquímica
/
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

DOI:
10.3791/54527-v

•

08:32 min

•

October 23, 2016

•

, , ,
1Department of Molecular, Cellular, and Developmental Biology,University of Michigan, 2Howard Hughes Medical Institute,University of Michigan

Capítulos

  • 00:05Título
  • 01:16Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
  • 03:18Effect of HdeB Overexpression on E. coli Survival under Acid Stress
  • 05:14Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
  • 07:16Conclusion

Summary

Traducción Automática

Traducción Automática

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

Tags

Escherichia ColiChaperone HdeBPH-dependent ActivityIn VitroIn VivoAcid Activated ChaperonesProtein UnfoldingProtein DegradationEnteric BacteriaStomachAcid Protective ChaperonesHDAMalate DehydrogenaseLight ScatteringFluorescent SpectrophotometerTemperature Control

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Quantification of Hypopigmentation Activity In Vitro

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Read Article