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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
1Department of Renewable Resources,University of Alberta, 2Department of Science, Augustana Faculty,University of Alberta, 3Laboratoire Génie Civil et géo-Environnement,Université de Lille, 4Department of Earth and Environmental Sciences,Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre,Natural Resources Canada
Capítulos
- 00:05Título
- 00:45Phospholipid Fatty Acid (PLFA) Technique: Extraction
- 03:14PLFA Technique: Lipid Fractionation
- 05:18PLFA Technique: Lipid Methylation
- 07:34Results: Microbial Phospholipid Fatty Acids in Soils
- 09:08Conclusion
Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.
