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Environment

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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
 

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Article DOI: 10.3791/54360-v 10:03 min August 26th, 2016
August 26th, 2016

章节

总结概括

Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.

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Keywords: Microbial Phospholipid Fatty Acids Soil Microbial Communities Land Management Changes Natural Disturbance Bligh And Dyer Extraction 19:0 Nonadeconoate Surrogate Standard Chloroform Methanol Citrate Buffer Centrifugation Nitrogen Evaporation Sample Storage
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