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Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
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August 26, 2016
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1Department of Renewable Resources,University of Alberta, 2Department of Science, Augustana Faculty,University of Alberta, 3Laboratoire Génie Civil et géo-Environnement,Université de Lille, 4Department of Earth and Environmental Sciences,Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre,Natural Resources Canada
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Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.
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Quideau, S. A., McIntosh, A. C., Norris, C. E., Lloret, E., Swallow, M. J., Hannam, K. Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils. J. Vis. Exp. (114), e54360, doi:10.3791/54360 (2016).
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