This content is Free Access.
Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
29,811 Views
•
10:03 min
•
August 26, 2016
DOI:
1Department of Renewable Resources,Alberta Üniversitesi, 2Department of Science, Augustana Faculty,Alberta Üniversitesi, 3Laboratoire Génie Civil et géo-Environnement,Université de Lille, 4Department of Earth and Environmental Sciences,Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre,Natural Resources Canada
Özet
Automatically generated
Phospholipid fatty acids provide information about the structure of soil microbial communities. We present methods for extraction from soil samples with a single-phase chloroform mixture, fractionation of extracted lipids using solid phase extraction columns, and methanolysis to produce fatty acid methyl esters, which are analyzed by capillary gas chromatography.
Read Article
Cite this Article
Quideau, S. A., McIntosh, A. C., Norris, C. E., Lloret, E., Swallow, M. J., Hannam, K. Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils. J. Vis. Exp. (114), e54360, doi:10.3791/54360 (2016).
Copy