/
/
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
This content is Free Access.
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
23,663 Views
•
08:29 min
•
February 06, 2015
DOI:
Summary
Automatically generated
The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.
Read Article
Cite this Article
Kummari, E., Guo-Ross, S. X., Eells, J. B. Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area. J. Vis. Exp. (96), e52336, doi:10.3791/52336 (2015).
Copy