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Journal / Neuroscience / Laser Capture Microdissection - A Demonstration…
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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
 

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Article DOI: 10.3791/52336-v • 08:29 min • February 6th, 2015
February 6th, 2015 •
1, 1, 1
1Department of Basic Sciences, Mississippi State University College of Veterinary Medicine

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The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.

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Laser Capture Microdissection LCM Isolation Individual Cells Anatomical Regions Tissue Sections Microscope Slide Immunohistochemistry Dopamine Neurons Ventral Tegmental Area Tyrosine Hydroxylase IR Capture Laser UV Cutting Laser PEN Membrane Slide
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