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Inmunología y contagio
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Isolation of Double Negative αβ T Cells from the Kidney
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation of Double Negative αβ T Cells from the Kidney

Isolation of Double Negative αβ T Cells from the Kidney

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06:56 min

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May 16, 2014

DOI:

10.3791/51192-v

DOI:
10.3791/51192-v

•

06:56 min
May 16, 2014

•

3 Views
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1Department of Pathology,Johns Hopkins University School of Medicine, 2Department of Medicine,Johns Hopkins University School of Medicine

Summary

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DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

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Martina, M. N., Bandapalle, S., Rabb, H., Hamad, A. R. Isolation of Double Negative αβ T Cells from the Kidney. J. Vis. Exp. (87), e51192, doi:10.3791/51192 (2014).

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