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Isolation of Double Negative αβ T Cells from the Kidney
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Isolation of Double Negative αβ T Cells from the Kidney

Isolation of Double Negative αβ T Cells from the Kidney

DOI:
10.3791/51192-v

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06:56 min

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May 16, 2014

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, , ,
1Department of Pathology,Johns Hopkins University School of Medicine, 2Department of Medicine,Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 01:47Kidney Mononuclear Cell (KMNC) Isolation
  • 03:48DN T Cell Isolation from CD45+ KMNC
  • 05:26Results: Representative Kidney DN αβ T Cell Purity
  • 06:31Conclusion

Summary

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DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.

Tags

Keywords: Double Negative T CellsDN T CellsKidneyIsolation ProtocolCD45+ SelectionNegative SelectionCD4CD8MHC Class IICD1d α-galcer TetramerPurity

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