Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Using Fluorescent Proteins to Visualize and…
JoVE Journal
Immunology and Infection

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
 

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Article DOI: 10.3791/51131-v • 07:42 min • October 13th, 2015
October 13th, 2015 •
1,2, 2, 1
1Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, 2Program in Infectious Diseases, School of Public Health, University of California at Berkeley

Capítulos

Resumen
Automatic Translation

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Tags

Keywords: Chlamydia Inclusion Vacuole Fluorescent Proteins GFP MCherry Visualization Quantitation Host Cell Intracellular Imaging Reverse-imaging Antibody-based Enumeration
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter