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鼠神经母细胞核转染到研究神经细胞迁移<em>在体外</em

JoVE Revista
Neurociencias

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JoVE Revista Neurociencias

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

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鼠神经母细胞核转染到研究神经细胞迁移<em>在体外</em

11,193 Views

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11:32 min

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November 12, 2013

DOI:

10.3791/50989-v

DOI:

10.3791/50989-v

•

11:32 min

November 12, 2013

•

1 Views

Katarzyna Falenta*¹, Sangeetha Gajendra*², Martina Sonego¹, Patrick Doherty¹, Giovanna Lalli¹

¹Wolfson Centre for Age-Related Diseases,King’s College London, ²MRC Centre for Developmental Neurobiology,King’s College London

Summary

成神经细胞迁移是在出生后的神经发生了关键性的一步。在这里描述的协议可以被用于研究神经细胞迁移的候选调节器通过使用DNA /小发夹RNA（shRNA的）核转染，并与成神经细胞从啮齿动物出生后的头端迁移流中分离出来的三维迁移实验中的作用。

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