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재조합 Adeno - 관련 바이러스성 벡터의 생산 및 Titering
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Production and Titering of Recombinant Adeno-associated Viral Vectors
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재조합 Adeno - 관련 바이러스성 벡터의 생산 및 Titering

60,834 Views

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08:35 min

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November 27, 2011

DOI:

10.3791/3348-v

DOI:
10.3791/3348-v

•

08:35 min
November 27, 2011

•

23 Views
, , , ,
1School of Medical Sciences, College of Life Sciences and Medicine,University of Aberdeen, 2Translational Neuroscience Facility and Department of Physiology,School of Medical Sciences, University of New South Wales, 3Department of Biochemistry and Molecular Biophysics,Columbia University

Summary

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재조합 adeno - 관련 바이러스 (rAAVs) 벡터를 위해 점점 중요 해지고 있습니다<em> 생체내에</em> 동물의 연구. 우리는 rAAVs는 실험실에서 생산되는 수 있으며, 어떻게 이러한 벡터가 생성 전염성 입자의 수​​를 정확하게 읽기를 제공 titered 수있는 방법에 대해 설명합니다.

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McClure, C., Cole, K. L. H., Wulff, P., Klugmann, M., Murray, A. J. Production and Titering of Recombinant Adeno-associated Viral Vectors. J. Vis. Exp. (57), e3348, doi:10.3791/3348 (2011).

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