Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Título
- 01:32Brain Slice Preparation
- 02:17Equipment Startup
- 03:24Whole Cell Patch-clamp Recording
- 05:52Laser Scanning Photostimulation
- 07:44Representative Results for Electrophysiological Recording and Photostimulation Mapping
This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.
Tags
Inhibitory NeuronsNeuronal CircuitsLaser Scanning PhotostimulationCortical FunctionSubtypesImmunochemical PropertiesMorphological PropertiesPhysiological PropertiesLocal Circuit ConnectionsExcitatory Synaptic InputInhibitory Synaptic InputLaser Scanning Photostimulation (LSPS)Electrical StimulationGlutamate Puff StimulationMapping ResolutionCortical Circuit AnalysisWhole-cell Patch Clamping
