Journal
/
Inmunología y contagio
/
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

DOI:
10.3791/2659-v

•

08:50 min

•

June 02, 2011

•

, , , ,
1Institute for Molecular and Clinical Immunology,Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation,Helmholtz Center for Infection Research

Capítulos

  • 00:05Título
  • 01:29Spore Preparation
  • 02:54Infection
  • 04:05Lung Preparation
  • 06:332-Photon Laser Microscopic Visualization of Neutrophils and NETs in Control and Aspergillus fumigatis-infected Lung Tissue
  • 08:29Conclusion

Summary

Traducción Automática

Traducción Automática

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

Tags

PhagocytosisNET-formationNeutrophilsInfected Lungs2-photon MicroscopyGas ExchangeDefense StrategiesHumoral Immune MechanismsCellular Immune MechanismsRecruitment Of NeutrophilsCytotoxic ChemicalsExtracellular DNA-NETsBacteriaFungiLung InfectionViable Lung SlicesIntratracheal InfectionAspergillus FumigatusMulticolor Time-lapse Microscopy

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/2659?article_type=v

Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
check_url/es/2659?article_type=v

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
check_url/es/2659?article_type=v

Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
check_url/es/2659?article_type=v

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
check_url/es/2659?article_type=v

Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy
check_url/es/2659?article_type=v

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
check_url/es/2659?article_type=v

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
check_url/es/2659?article_type=v

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
check_url/es/2659?article_type=v

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
check_url/es/2659?article_type=v

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Read Article