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Microarray Analysis for Saccharomyces cerevisiae
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Biología
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JoVE Revista Biología
Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

DOI:
10.3791/2585-v

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13:17 min

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April 07, 2011

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, , ,
1Vermont Genetics Network,The University of Vermont

Capítulos

  • 00:05Título
  • 02:18Extracting Total RNA from Yeast Cultures
  • 05:03Microarray Target Preparation
  • 09:44Results of Microarray Analysis
  • 10:56Conclusion

Summary

Traducción Automática

Traducción Automática

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H2O2), an oxidizing agent.

Tags

Microarray AnalysisSaccharomyces CerevisiaeGene ExpressionOxidative StressHydrogen PeroxideYeast CultureGlucoseControl GroupTreated GroupHanks Buffered SalineTotal RNA ExtractionBiotin-labeled CRNAUVM Microarray Core FacilityAffymetrix Yeast GeneChipsGene Expression Data Analysis

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