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Microarray Analysis for Saccharomyces cerevisiae
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JoVE Journal 生物学
Microarray Analysis for Saccharomyces cerevisiae

Microarray Analysis for Saccharomyces cerevisiae

DOI:
10.3791/2585-v

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13:17 min

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April 07, 2011

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, , ,
1Vermont Genetics Network,The University of Vermont

章

  • 00:05標題
  • 02:18Extracting Total RNA from Yeast Cultures
  • 05:03Microarray Target Preparation
  • 09:44Results of Microarray Analysis
  • 10:56Conclusion

概要

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自動翻訳

In this protocol, gene expression in yeast (Saccharomyces cerevisiae) is changed after exposure to oxidative stress induced by the addition of hydrogen peroxide (H2O2), an oxidizing agent.

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Microarray AnalysisSaccharomyces CerevisiaeGene ExpressionOxidative StressHydrogen PeroxideYeast CultureGlucoseControl GroupTreated GroupHanks Buffered SalineTotal RNA ExtractionBiotin-labeled CRNAUVM Microarray Core FacilityAffymetrix Yeast GeneChipsGene Expression Data Analysis

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