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从多能干细胞生成人体血管类器官
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
DOI:

09:46 min

January 20, 2023

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Kapitel

  • 00:04Introduction
  • 00:52Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension
  • 02:34Day 1: Mesoderm Induction of the Aggregates
  • 03:41Day 4: Vascular Induction and Priming of the Aggregates
  • 04:20Day 6: Aggregate Embedding and Vessel Sprout Induction
  • 06:02Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)
  • 07:25Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs
  • 09:13Conclusion

Summary

Automatische Übersetzung

该协议描述了从人类多能和诱导多能干细胞产生自组织血管。这些血管网络表现出广泛且连接的内皮网络,周围环绕着周细胞、平滑肌肌动蛋白和连续的基底膜。

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