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Biotechnik
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从多能干细胞生成人体血管类器官
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal
Biotechnik
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
从多能干细胞生成人体血管类器官
DOI:
10.3791/64715-v
•
09:46 min
•
January 20, 2023
•
Nicolas Werschler
2
,
Josef Penninger
2,3,4
1
School of Biomedical Engineering
,
University of British Columbia
,
2
Life Sciences Institute
,
University of British Columbia
,
3
Department of Medical Genetics
,
University of British Columbia
,
4
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
Kapitel
00:04
Introduction
00:52
Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension
02:34
Day 1: Mesoderm Induction of the Aggregates
03:41
Day 4: Vascular Induction and Priming of the Aggregates
04:20
Day 6: Aggregate Embedding and Vessel Sprout Induction
06:02
Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)
07:25
Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs
09:13
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
该协议描述了从人类多能和诱导多能干细胞产生自组织血管。这些血管网络表现出广泛且连接的内皮网络,周围环绕着周细胞、平滑肌肌动蛋白和连续的基底膜。
Tags
Blood Vessel Organoids
Pluripotent Stem Cells
Vasculogenesis
Angiogenesis
Vascular Disease
Vascular Pathologies
Reproducibility
High Throughput
Stem Cell Lines
Diabetic Vasculopathy
Aggregates
Cell Dissociation
Aggregation Medium
Single-cell Suspension
Cell Viability
Mesoderm Media
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