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Neuroscience

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JoVE Journal Neuroscience
Modeling Human Cerebellar Development In Vitro in 2D Structure
 

Modeling Human Cerebellar Development In Vitro in 2D Structure

Article DOI: 10.3791/64462-v • 06:14 min • September 16th, 2022
September 16th, 2022 •
1,3,4,5, 1,3,4,5, 1,3, 1,2,3,4,5, 1,3,4,5, 1,3,4,5
1Department of Psychiatry, University of Iowa, 2Department of Radiology, University of Iowa, 3Carver College of Medicine, University of Iowa, 4Iowa Neuroscience Institute, University of Iowa, 5Pappajohn Biomedical Institute, University of Iowa

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Summary
Automatic Translation

The present protocol explains the generation of a 2D monolayer of cerebellar cells from induced pluripotent stem cells for investigating the early stages of cerebellar development.

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Human Cerebellar Development In Vitro 2D Structure Neuropsychiatric Disorders Cost Efficient Pull Pipettes Experimental Preparation Autoclave Sleeves Sterilize Cerebellar Differentiation Medium Y-27632 SB-431542 Six-well Ultra-low Attachment Plate Differentiated Cells IPSC Passaging Solution
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