/
/
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
7,912 Views
•
10:36 min
•
November 06, 2017
DOI:
Summary
Automatically generated
This protocol describes a simple method for isolating and culturing primary mouse cerebral granule neurons (CGNs) from 6-7 day old pups, efficient transduction of CGNs for loss and gain of function studies, and modelling NMDA-induced neuronal excitotoxicity, low-potassium-induced cell death, DNA-damage, and oxidative stress using the same culture model.
Read Article
Cite this Article
Laaper, M., Haque, T., Slack, R. S., Jahani-Asl, A. Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons. J. Vis. Exp. (129), e55871, doi:10.3791/55871 (2017).
Copy