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Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
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Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
Kapitel
- 00:05Introduction
- 00:39Preparing the Injection Syringe for Electroporation
- 01:00Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
- 02:34Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
- 03:45Conclusion
Here we describe a method to visualize synaptogenesis of granule neurons in the mouse cerebellum over the time course of postnatal brain development when these cells refine their synaptic structures and form synapses to integrate themselves into the overall brain circuit.
