Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:04Introduction
- 00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
- 01:31Laser Calibration
- 02:48Laser Ablation
- 04:21Monitoring the Growth of Irradiated Embryos
- 04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
- 05:46Conclusion
The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.
