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Neuroscience

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JoVE Journal Neuroscience
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
 

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

Article DOI: 10.3791/63327-v • 07:40 min • February 11th, 2022
February 11th, 2022 •
1, 2, 1,3,4
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science, Aalto University School of Science, 3HiLIFE - Neuroscience Center, University of Helsinki, 4HiLIFE - Institute of Biotechnology, University of Helsinki

Kapitel

Summary
Automatic Translation

The present protocol describes a method to visualize and measure actin rings and other components of the membrane periodic skeleton of the axon initial segment using cultured rat hippocampal neurons and 3D-structured illumination microscopy (3D-SIM).

Tags

Membrane Periodic Skeleton Axon Initial Segment 3D-Structured Illumination Microscopy (3D-SIM) Protein Localization Fluorescence Microscopy Sample Preparation Signal To Noise Ratio Fix Neurons Paraformaldehyde BSA Triton X Ankyrin G Antibodies Overnight Incubation Fluoro 4 Tagged Secondary Antibodies
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