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Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
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JoVE Journal Neurowissenschaften
Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

1,970 Views

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07:40 min

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February 11, 2022

DOI:

10.3791/63327-v

DOI:
10.3791/63327-v

•

07:40 min
February 11, 2022

•

7 Views
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1Minerva Foundation Institute for Medical Research, 2Department of Computer Science,Aalto University School of Science, 3HiLIFE – Neuroscience Center,University of Helsinki, 4HiLIFE – Institute of Biotechnology,University of Helsinki

Summary

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The present protocol describes a method to visualize and measure actin rings and other components of the membrane periodic skeleton of the axon initial segment using cultured rat hippocampal neurons and 3D-structured illumination microscopy (3D-SIM).

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3D-SIMMembrane Periodic SkeletonAxon Initial SegmentFluorescence MicroscopySample PreparationAnkyrin GFluoro 4FerodinMountingImmersion Oil

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Micinski, D., Lahti, L., Abouelezz, A. Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM). J. Vis. Exp. (180), e63327, doi:10.3791/63327 (2022).

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