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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
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JoVE Journal Neurowissenschaften
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

15,632 Views

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14:11 min

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May 04, 2014

DOI:

10.3791/51276-v

DOI:
10.3791/51276-v

•

14:11 min
May 04, 2014

•

8 Views
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1Center for Neuroscience, Swammerdam Institute for Life Sciences,University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences,University of Amsterdam

Summary

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This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.

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Cite this Article

Schouten, M., De Luca, G. M. R., Alatriste González, D. K., de Jong, B. E., Timmermans, W., Xiong, H., Krugers, H., Manders, E. M. M., Fitzsimons, C. P. Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy. J. Vis. Exp. (87), e51276, doi:10.3791/51276 (2014).

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