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Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion
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JoVE Journal Neurowissenschaften
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

DOI:
10.3791/58171-v

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06:17 min

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September 01, 2018

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, ,
1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Kapitel

  • 00:04Titel
  • 00:38Surgical Exposure of the L4 and L5 DRGs
  • 01:37DRG Injection and Electroporation
  • 02:23Sciatic Nerve Crush
  • 03:07DRG and Sciatic Nerve Harvest and Imaging
  • 04:23Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
  • 05:35Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Electroporation is an effective approach to deliver genes of interests into cells. By applying this approach in vivo on the neurons of adult mouse dorsal root ganglion (DRG), we describe a model to study axon regeneration in vivo.

Tags

In Vivo ElectroporationAdult Mouse Dorsal Root GanglionGene TransfectionAxon RegenerationSensory NeuronsSciatic Nerve CrushNerve InjuryGene OverexpressionGene Knockdown

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