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从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案

6,959 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

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04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

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在这里，我们提出了一种经济有效的方法，在用10 ng / mL GM-CSF / IL-4培养7天后从小鼠中分离和产生高纯度骨髓来源的树突状细胞。

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Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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Cite this Article

Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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