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Immunologie und Infektion
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在生物安全3级(BSL-3)条件下使胃肠道类器官适应病原体感染和单细胞测序
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal
Immunologie und Infektion
Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
Please note that all translations are automatically generated.
Click here for the English version.
在生物安全3级(BSL-3)条件下使胃肠道类器官适应病原体感染和单细胞测序
DOI:
10.3791/62857-v
•
07:59 min
•
September 10, 2021
•
Megan L. Stanifer
2
,
Steeve Boulant
3,4
1
Department of Infectious Diseases, Molecular Virology
,
Heidelberg University Hospital
,
2
Department of Molecular Genetics and Microbiology, College of Medicine
,
University of Florida, Gainesville
,
3
Department of Infectious Diseases, Virology
,
Heidelberg University Hospital
,
4
Research Group “Cellular Polarity and Viral Infection”
,
German Cancer Research Center (DKFZ)
Kapitel
00:04
Introduction
01:04
Preparation of Single-Cell Solution and Preparation of Gel Beads-in-Emulsion Under Biosafety Level 3 Conditions
05:47
Results: Characterization of Host/Pathogen Interactions at a Single-Cell Level Using scRNAseq Technology
07:11
Conclusion
Summary
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Automatische Übersetzung
该协议描述了如何从根尖或基底外侧感染人肠道类器官,以使用单细胞RNA测序(scRNAseq)技术在单细胞水平上表征宿主/病原体相互作用。
Tags
Gastrointestinal Organoids
Pathogen Infection
Single Cell Sequencing
Biosafety Level 3
Viral Infection
Intestinal Epithelium
Differentiated Organoids
Extracellular Matrix
Dissociation Enzyme
Single Cell RNA Sequencing
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