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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

DOI:

07:59 min

September 10, 2021

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Chapters

  • 00:04Introduction
  • 01:04Preparation of Single-Cell Solution and Preparation of Gel Beads-in-Emulsion Under Biosafety Level 3 Conditions
  • 05:47Results: Characterization of Host/Pathogen Interactions at a Single-Cell Level Using scRNAseq Technology
  • 07:11Conclusion

Summary

自动翻译

This protocol describes how to infect human intestinal organoids from either their apical or basolateral side to characterize host/pathogen interactions at the single-cell level using single-cell RNA sequencing (scRNAseq) technology.

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