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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

2,603 Views

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07:59 min

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September 10, 2021

DOI:

10.3791/62857-v

DOI:

10.3791/62857-v

•

07:59 min

September 10, 2021

•

3 Views

Megan L. Stanifer², Steeve Boulant^3,4

¹Department of Infectious Diseases, Molecular Virology,Heidelberg University Hospital, ²Department of Molecular Genetics and Microbiology, College of Medicine,University of Florida, Gainesville, ³Department of Infectious Diseases, Virology,Heidelberg University Hospital, ⁴Research Group “Cellular Polarity and Viral Infection”,German Cancer Research Center (DKFZ)

Tags

Gastrointestinal Organoids Pathogen Infection Single Cell Sequencing Biosafety Level 3 Viral Infection Intestinal Epithelium Differentiated Organoids Extracellular Matrix Dissociation Enzyme Single Cell RNA Sequencing

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