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荧光钙成像及其后续原位杂交，用于异种性前体特征
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
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荧光钙成像及其后续原位杂交，用于异种性前体特征

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00:09 min

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February 18, 2020

DOI:

10.3791/60726-v

DOI:
10.3791/60726-v

•

00:09 min
February 18, 2020

•

3 Views
, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Department of Biology,College of William and Mary, 3Harvard Medical School,Harvard University, 4Department of Bioengineering,California Institute of Technology, 5Department of Computer Science,College of William and Mary

Summary

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我们提出了一个两部分的协议，将荧光钙成像与原位杂交相结合，使实验者能够将钙活性模式与单细胞水平上的基因表达谱相关联。

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Cite this Article

Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).

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