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Characterizing Mutational Load and Clonal Composition of Human Blood
JoVE Journal
Genetik
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JoVE Journal Genetik
Characterizing Mutational Load and Clonal Composition of Human Blood

Characterizing Mutational Load and Clonal Composition of Human Blood

DOI:
10.3791/59846-v

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07:58 min

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July 11, 2019

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, , ,
1Princess Máxima Center for Pediatric Oncology

Kapitel

  • 00:04Titel
  • 00:41HSPC Isolation, Sorting, and Culture
  • 02:29Harvesting HSPC Clones
  • 04:05Construction of a Developmental Lineage Tree Using Base Substitutions
  • 06:05Results: Checking Quality After Sequencing and Mutation Analysis, and Constructing a Developmental Lineage Tree
  • 07:38Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Somatic mutation patterns in cells reflect previous mutagenic exposure and can reveal developmental lineage relationships. Presented here is a methodology to catalogue and analyze somatic mutations in individual hematopoietic stem and progenitor cells.

Tags

Mutational LoadClonal CompositionHematopoietic Stem CellDNA RepairMutagenicCell SortingHSPCCD34CD38CD45RACloneCell CultureSingle Cell

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