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原代小鼠骨骼肌微血管内皮细胞的分离
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JoVE Journal Neurowissenschaften
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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原代小鼠骨骼肌微血管内皮细胞的分离

9,877 Views

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11:57 min

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March 06, 2019

DOI:

10.3791/58901-v

DOI:
10.3791/58901-v

•

11:57 min
March 06, 2019

•

25 Views
, , , ,
1Institute for Translational Neurology and Neurology Clinic,University of Muenster

Summary

Automatically generated

骨骼肌微血管内皮细胞 (mmec) 塑造了肌肉毛细血管的内壁, 并调节两者, 流体分子的交换和 (免疫) 细胞在肌肉组织和血液之间的迁移。分离原生小鼠 mmeec, 如下所述, 使 "我的血管单位" 能够进行全面的体外调查。

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Primary MurineSkeletal MuscleMicrovascular Endothelial CellsIsolationCell CultureDigestion

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Cite this Article

Müntefering, T., Michels, A. P., Pfeuffer, S., Meuth, S. G., Ruck, T. Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells. J. Vis. Exp. (145), e58901, doi:10.3791/58901 (2019).

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