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marinum 分枝杆菌感染成人斑马鱼结核的建模
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
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marinum 分枝杆菌感染成人斑马鱼结核的建模

10,262 Views

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07:00 min

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October 08, 2018

DOI:

10.3791/58299-v

DOI:
10.3791/58299-v

•

07:00 min
October 08, 2018

•

5 Views
, , ,
1Faculty of Medicine and Life Sciences,University of Tampere, 2Oral and Maxillofacial Unit,Tampere University Hospital

Summary

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在这里, 我们提出了一个在成年斑马鱼使用其天然致病菌分枝杆菌 marinum的人结核病模型的协议。从被感染的斑马鱼内脏中提取的 DNA 和 RNA 可以用来揭示鱼的总结核杆菌负荷和宿主对 qPCR 的免疫应答。

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Luukinen, H., Hammarén, M. M., Vanha-aho, L., Parikka, M. Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish. J. Vis. Exp. (140), e58299, doi:10.3791/58299 (2018).

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