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MALDI-TOF 質量分析法によるマウス組織におけるプロテアーゼの特異性の定量抽出: 特異性変化を引き起こすに PH を操作します。
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Biochemie
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JoVE Journal Biochemie
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
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MALDI-TOF 質量分析法によるマウス組織におけるプロテアーゼの特異性の定量抽出: 特異性変化を引き起こすに PH を操作します。

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09:47 min

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May 25, 2018

DOI:

10.3791/57469-v

DOI:
10.3791/57469-v

•

09:47 min
May 25, 2018

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2 Views
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1Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

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Protease SpecificityMALDI-TOF Mass SpectrometryPH ManipulationProtease AssayBiotin LabelingStreptavidin Beads

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Yamamoto, H., Sawaguchi, Y., Kimura, M. The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes. J. Vis. Exp. (135), e57469, doi:10.3791/57469 (2018).

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