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質量分析を用いた識別 RNA 結合領域のための試料作製

JoVE Journal
Biochemie

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JoVE Journal Biochemie

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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質量分析を用いた識別 RNA 結合領域のための試料作製

7,936 Views

•

10:52 min

•

September 28, 2017

DOI:

10.3791/56004-v

DOI:

10.3791/56004-v

•

10:52 min

September 28, 2017

•

1 Views

Robert Warneford-Thomson⁴, Chongsheng He², Simone Sidoli³, Benjamin A. Garcia³, Roberto Bonasio²

¹Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, ²Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ³Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, ⁴Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

Tags

Sample Preparation Mass Spectrometry RNA-binding Proteins UV Cross-linking 4sU Labeling Cell Fractionation Immunoprecipitation Protein-RNA Interactions Epigenetics Cell Culture Mouse Embryonic Stem Cells

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