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Kronik Lenfositik Lösemi Hastalarında Metil-CpG-Bağlayıcı Alan Yakalamaya Dayalı Bir Yöntem Kullanarak Kapsamlı DNA Metilasyon Analizi
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JoVE Journal Genetik
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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Kronik Lenfositik Lösemi Hastalarında Metil-CpG-Bağlayıcı Alan Yakalamaya Dayalı Bir Yöntem Kullanarak Kapsamlı DNA Metilasyon Analizi

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13:21 min

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June 16, 2017

DOI:

10.3791/55773-v

DOI:
10.3791/55773-v

•

13:21 min
June 16, 2017

•

4 Views
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1Department of Medical Genetics, Institute of Biomedicine,Gothenburg University, 2Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine,Gothenburg University

Summary

Automatically generated

Bu çalışma, kronik lenfositik lösemi (KL) hastalarında farklı şekilde metilasyona uğramış CpG bakımından zengin bölgelerin tanımlanması için optimize edilmiş bir metil-CpG-bağlanma alanı (MBD) sıralama protokolünü ve bir hesaplama hattını açıklamaktadır.

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Cite this Article

Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).

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