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Journal / Biochemistry / Brain Membrane Fractionation: An Ex Vivo Approach …
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Biochemistry
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JoVE Journal Biochemistry
Brain Membrane Fractionation
 

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Article DOI: 10.3791/55661-v • 09:49 min • May 12th, 2017
May 12th, 2017 •
*1,2, *1,2, 3, 3, 1,2
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.

Tags

Brain Membrane Fractionation Ex Vivo Approach Subsynaptic Protein Localization Synaptic Compartments Kinetic Transmission Components Synaptic Function Electron Microscopy Membrane Fractionation Method Laser Obliteration Mild Detergents PH Conditions Centrifugation Hippocampus Dissection Isolation Buffer Homogenizing Stirrer Sucrose Calcium Solution Synaptosomes Sub-synaptic Compartments
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