Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
10,213 Views
•
09:49 min
•
May 12, 2017
DOI:
1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L’Hospitalet de Llobregat, 2Institut de Neurociències,Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra
Summary
Automatically generated
Here, we present a brain membrane fractionation protocol that represents a robust procedure to isolate proteins belonging to different synaptic compartments.
Read Article
Cite this Article
Morató, X., López-Cano, M., Canas, P. M., Cunha, R. A., Ciruela, F. Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization. J. Vis. Exp. (123), e55661, doi:10.3791/55661 (2017).
Copy