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Maturazione di staminali umane cardiomiociti derivati ​​dalle cellule in Biowires Utilizzando Stimolazione Elettrica
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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Maturazione di staminali umane cardiomiociti derivati ​​dalle cellule in Biowires Utilizzando Stimolazione Elettrica

8,967 Views

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10:11 min

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May 06, 2017

DOI:

10.3791/55373-v

DOI:
10.3791/55373-v

•

10:11 min
May 06, 2017

•

1 Views
,
1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Summary

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La piattaforma BIOWIRE cardiaco è un metodo in vitro utilizzato per maturare embrionale umano e cardiomiociti derivati dalle cellule staminali pluripotenti indotte (HPSC-CM) combinando coltura cellulare tridimensionale con stimolazione elettrica. Questo manoscritto presenta la configurazione dettagliata della piattaforma BIOWIRE cardiaco.

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Cite this Article

Sun, X., Nunes, S. S. Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation. J. Vis. Exp. (123), e55373, doi:10.3791/55373 (2017).

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