Journal
/
Biologia do Desenvolvimento
/
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

8,968 Views

•

10:11 min

•

May 06, 2017

DOI:

10.3791/55373-v

DOI:
10.3791/55373-v

•

10:11 min
May 06, 2017

•

1 Views
,
1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Summary

Automatically generated

The cardiac biowire platform is an in vitro method used to mature human embryonic and induced pluripotent stem cell-derived cardiomyocytes (hPSC-CM) by combining three-dimensional cell cultivation with electrical stimulation. This manuscript presents the detailed setup of the cardiac biowire platform.

Explore More Videos

Stem CellCardiomyocyteMaturationElectrical StimulationBiowireDrug ScreeningPDMSSuturePolycarbonateCarbon RodPlatinum Wire

Read Article

Cite this Article

Sun, X., Nunes, S. S. Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation. J. Vis. Exp. (123), e55373, doi:10.3791/55373 (2017).

Download .ris file

Copy

Share Video

.

Copy