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Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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发育生物学
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JoVE 杂志 发育生物学
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

8,961 Views

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10:11 min

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May 06, 2017

DOI:

10.3791/55373-v

DOI:
10.3791/55373-v

•

10:11 min
May 06, 2017

•

1 Views
,
1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Summary

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The cardiac biowire platform is an in vitro method used to mature human embryonic and induced pluripotent stem cell-derived cardiomyocytes (hPSC-CM) by combining three-dimensional cell cultivation with electrical stimulation. This manuscript presents the detailed setup of the cardiac biowire platform.

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Cite this Article

Sun, X., Nunes, S. S. Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation. J. Vis. Exp. (123), e55373, doi:10.3791/55373 (2017).

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