Journal
/
Entwicklungsbiologie
/
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

DOI:
10.3791/55373-v

•

10:11 min

•

May 06, 2017

•

,
1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Kapitel

  • 00:05Titel
  • 01:01Fabrication of Biowire Templates
  • 02:22Fabricating the Electrical Simulation Chambers
  • 03:55Gel Preparation and Seeding of Cardiomyocytes into the Template
  • 05:41Culturing Cardiomyocytes in the Template with Electrical Stimulation (Biowire)
  • 07:22Results: Biowire Cardiomyocyte Physiology
  • 09:16Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The cardiac biowire platform is an in vitro method used to mature human embryonic and induced pluripotent stem cell-derived cardiomyocytes (hPSC-CM) by combining three-dimensional cell cultivation with electrical stimulation. This manuscript presents the detailed setup of the cardiac biowire platform.

Tags

Stem CellCardiomyocyteMaturationElectrical StimulationBiowireDrug ScreeningPDMSSuturePolycarbonateCarbon RodPlatinum Wire

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

Read Article